- 2D experiment in 96-well plate
- Cell line: PANC-1 7500 cells/well; P47; viability 69 %
- Nanoparticles: GGAG:Ce@SiO2,
GGAG:Ce@SiO2-RB
- Concentration: 0.0; 0.05; 0.5; 5.0; 50; 500; 1000 µg/mL
- Other conditions:
- total killing with 10% DMSO (6 wells)
- blank without MTS solution (6 wells)
- covering the plate with aluminum foil all the time (inclusive of
incubation)
- Growing time approx. 26h
- Incubation time 23h
- MTS incubation 3h
- R1 first replication
Experiment procedure
- 96-well plate, seeding 7500 cells/well in 100 µL culture medium
- Growing from: 12.12. 9:25; about 26h
- Removing medium
- Absorbance measurement at 485 nm (cells without any nano or
medium)
- Adding 100 µL nanoparticle’s suspension (suspension prepared before
adding)
- Covering with aluminum foil and putting to incubator
- Incubation from: 13.12. 12:15; about 23h
- Rinsing cells with PBS
- Absorbance measurement at 485 nm
- Adding 80 µL of medium + 20 µL CellTiter Aqueous One Solution Cell
Proliferation Assay
- 3 hours incubation
- Absorbance measurement at 485 nm
- Putting 80 µL of the viability solution to new 96-well plate for
measurement without cells
- Removing the rest of viability solution from the previous plate
- Absorbance measurement at 485 nm both plates
Data processing
- count average of the blank wells (MTS solution without cells)
- BLANK CORRECTED: subtract the average of the blank from each
well
- count average of control wells (cells without nano) = 100 %
viability
- VIABILITY %: count % viability for each well
- count average % viability for each condition
- count standard deviation of the sample (function STDEV.S)
Data
Measurement without
nanoparticles
MTS
solution; 3h MTS incubation
MTS
solution; 3h MTS incubation
Viability table for all data
